The polymerase chain reaction pcr is the most sensitive method for revealing the presence of otherwise undetectable quantities of the genome of rna or dna of human viruses the polymerase chain reaction pcr for human viral diagnosis addresses the urgent need to use this revolutionary technology in reference and routine diagnostic laboratories. Polymerase chain reaction pcr is a broadly applied laboratory test for the diagnosis of a wide variety of central nervous system cns diseases including genetic and autoimmune diseases malignant neoplasms and infections 12 with its ability to detect minute amounts of dna or rna contained in tissues or fluids pcr has improved the rapidity and accuracy of diagnosis enhanced . Polymerase chain reaction for rubella virus diagnosis william f carman and elizabeth a b mccruden ch 10 pcr technology for lyssavirus diagnosis noel tordo herve bourhy and debora sacramento ch 11 pcr methods in the diagnosis of hiv 1 and hiv 2 infection peter balfe ch 12. Polymerase chain reaction is a highly sensitive technique for the detection of hepatitis b virus dna and hepatitis c virus rna in serum liver tissue and peripheral mononuclear blood cells in chronic hepatitis b it is particularly useful for identification of infectious subjects who are hepatitis . Polymerase chain reaction pcr is a technique that is used to amplify trace amounts of dna and in some instances rna located in or on almost any liquid or surface where dna strands may be deposited the key to understanding pcr is to know that every human animal plant parasite bacterium or virus contains genetic material such as dna
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